BD HorizonTM BB515 Rat Anti-Mouse CD45

Description

Flow cytometric analysis of CD45 expression on mouse splenocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG2b, kappa Isotype Control Cat. No. 564421; dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD45 antibody Cat. No. 564590; bold solid line histogram . Alternatively, cells were stained with FITC Anti-Mouse CD45 antibody Cat. No. 553079/553080/561088; thin solid line histogram . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD45 antibody versus its Ig Isotype Control Left Panel , and BB515 Anti-CD45 antibody versus FITC Anti-CD45 antibody Right Panel . The fluorescence histograms showing CD45 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD45 expression on mouse splenocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG2b, kappa Isotype Control Cat. No. 564421; dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD45 antibody Cat. No. 564590; bold solid line histogram . Alternatively, cells were stained with FITC Anti-Mouse CD45 antibody Cat. No. 553079/553080/561088; thin solid line histogram . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD45 antibody versus its Ig Isotype Control Left Panel , and BB515 Anti-CD45 antibody versus FITC Anti-CD45 antibody Right Panel . The fluorescence histograms showing CD45 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD45 expression on mouse splenocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG2b, kappa Isotype Control Cat. No. 564421; dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD45 antibody Cat. No. 564590; bold solid line histogram . Alternatively, cells were stained with FITC Anti-Mouse CD45 antibody Cat. No. 553079/553080/561088; thin solid line histogram . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD45 antibody versus its Ig Isotype Control Left Panel , and BB515 Anti-CD45 antibody versus FITC Anti-CD45 antibody Right Panel . The fluorescence histograms showing CD45 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD45 expression on mouse splenocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG2b, kappa Isotype Control Cat. No. 564421; dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD45 antibody Cat. No. 564590; bold solid line histogram . Alternatively, cells were stained with FITC Anti-Mouse CD45 antibody Cat. No. 553079/553080/561088; thin solid line histogram . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD45 antibody versus its Ig Isotype Control Left Panel , and BB515 Anti-CD45 antibody versus FITC Anti-CD45 antibody Right Panel . The fluorescence histograms showing CD45 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD45 expression on mouse splenocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG2b, kappa Isotype Control Cat. No. 564421; dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD45 antibody Cat. No. 564590; bold solid line histogram . Alternatively, cells were stained with FITC Anti-Mouse CD45 antibody Cat. No. 553079/553080/561088; thin solid line histogram . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD45 antibody versus its Ig Isotype Control Left Panel , and BB515 Anti-CD45 antibody versus FITC Anti-CD45 antibody Right Panel . The fluorescence histograms showing CD45 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD45 expression on mouse splenocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG2b, kappa Isotype Control Cat. No. 564421; dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD45 antibody Cat. No. 564590; bold solid line histogram . Alternatively, cells were stained with FITC Anti-Mouse CD45 antibody Cat. No. 553079/553080/561088; thin solid line histogram . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD45 antibody versus its Ig Isotype Control Left Panel , and BB515 Anti-CD45 antibody versus FITC Anti-CD45 antibody Right Panel . The fluorescence histograms showing CD45 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.