BD HorizonTM BB515 Rat Anti-Mouse CD62L

Description

Flow cytometric analysis of mouse CD62L expression - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Left Panel - CD62L expression on mouse bone marrow cells. Mouse bone marrow cells were untreated Left Plot or cultured 1 hr with Phorbol 12-Myristate 13-Acetate PMA; Middle Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . They were then stained with either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD62L antibody Cat. No. 565261; bold solid line histogram . Alternatively, untreated cells Right Plot were stained with FITC Anti-Mouse CD62L antibody Cat. No. 553150/561917; thin solid line histogram . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD62L antibody versus its Ig Isotype Control Left Plot , and BB515 Anti-CD62L antibody versus FITC Anti-CD62L antibody Right Plot . The fluorescence histograms showing CD62L expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells using a BD LSR II Flow Cytometer System. Right Panel - CD62L expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Left Plot , BD Horizon BB515 Rat Anti-Mouse CD62L antibody Middle Plot , or FITC Anti-Mouse CD62L antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD62L or Ig Isotype control staining versus CD3e were derived from gated events with the light-scatter characteristics of viable leucocytes using a BD LSR II Flow Cytometer System. Flow cytometric analysis of mouse CD62L expression - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Left Panel - CD62L expression on mouse bone marrow cells. Mouse bone marrow cells were untreated Left Plot or cultured 1 hr with Phorbol 12-Myristate 13-Acetate PMA; Middle Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . They were then stained with either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD62L antibody Cat. No. 565261; bold solid line histogram . Alternatively, untreated cells Right Plot were stained with FITC Anti-Mouse CD62L antibody Cat. No. 553150/561917; thin solid line histogram . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD62L antibody versus its Ig Isotype Control Left Plot , and BB515 Anti-CD62L antibody versus FITC Anti-CD62L antibody Right Plot . The fluorescence histograms showing CD62L expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells using a BD LSR II Flow Cytometer System. Right Panel - CD62L expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Left Plot , BD Horizon BB515 Rat Anti-Mouse CD62L antibody Middle Plot , or FITC Anti-Mouse CD62L antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD62L or Ig Isotype control staining versus CD3e were derived from gated events with the light-scatter characteristics of viable leucocytes using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of mouse CD62L expression - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Left Panel - CD62L expression on mouse bone marrow cells. Mouse bone marrow cells were untreated Left Plot or cultured 1 hr with Phorbol 12-Myristate 13-Acetate PMA; Middle Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . They were then stained with either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD62L antibody Cat. No. 565261; bold solid line histogram . Alternatively, untreated cells Right Plot were stained with FITC Anti-Mouse CD62L antibody Cat. No. 553150/561917; thin solid line histogram . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD62L antibody versus its Ig Isotype Control Left Plot , and BB515 Anti-CD62L antibody versus FITC Anti-CD62L antibody Right Plot . The fluorescence histograms showing CD62L expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells using a BD LSR II Flow Cytometer System. Right Panel - CD62L expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Left Plot , BD Horizon BB515 Rat Anti-Mouse CD62L antibody Middle Plot , or FITC Anti-Mouse CD62L antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD62L or Ig Isotype control staining versus CD3e were derived from gated events with the light-scatter characteristics of viable leucocytes using a BD LSR II Flow Cytometer System.