BD HorizonTM BB515 Rat Anti-Mouse CD8a

Description

Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 561826/553066 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD8a antibody Cat. No. 564422/564459; Right Panel antibodies. The two-color fluorescence contour plots showing the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 561826/553066 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD8a antibody Cat. No. 564422/564459; Right Panel antibodies. The two-color fluorescence contour plots showing the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 561826/553066 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD8a antibody Cat. No. 564422/564459; Right Panel antibodies. The two-color fluorescence contour plots showing the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 561826/553066 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD8a antibody Cat. No. 564422/564459; Right Panel antibodies. The two-color fluorescence contour plots showing the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 561826/553066 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD8a antibody Cat. No. 564422/564459; Right Panel antibodies. The two-color fluorescence contour plots showing the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 561826/553066 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD8a antibody Cat. No. 564422/564459; Right Panel antibodies. The two-color fluorescence contour plots showing the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.