BD HorizonTM BB515 Rat Anti-Mouse IL-33 Receptor (ST2)

Description

Multicolor flow cytometric analysis of IL-33 Receptor ST2 expression by Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat No. 553141/553142 . The splenic leukocytes were then stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 followed by staining with BD Horizon BV711 Rat Anti-Mouse CD4 antibody Cat. No. 563050 and with either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Plot or BD Horizon BB515 Rat Anti-Mouse IL-33 Receptor ST2 antibody Cat. No. 570078/570159; Right Plot at 1 mug/test. The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 563486 . The bivariate pseudocolor density plot showing the correlated expression of Foxp3 versus IL-33 Receptor ST2 or Ig Isotype control staining was derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of IL-33 Receptor ST2 expression by Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat No. 553141/553142 . The splenic leukocytes were then stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 followed by staining with BD Horizon BV711 Rat Anti-Mouse CD4 antibody Cat. No. 563050 and with either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Plot or BD Horizon BB515 Rat Anti-Mouse IL-33 Receptor ST2 antibody Cat. No. 570078/570159; Right Plot at 1 mug/test. The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 563486 . The bivariate pseudocolor density plot showing the correlated expression of Foxp3 versus IL-33 Receptor ST2 or Ig Isotype control staining was derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of IL-33 Receptor ST2 expression by Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat No. 553141/553142 . The splenic leukocytes were then stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 followed by staining with BD Horizon BV711 Rat Anti-Mouse CD4 antibody Cat. No. 563050 and with either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Plot or BD Horizon BB515 Rat Anti-Mouse IL-33 Receptor ST2 antibody Cat. No. 570078/570159; Right Plot at 1 mug/test. The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 563486 . The bivariate pseudocolor density plot showing the correlated expression of Foxp3 versus IL-33 Receptor ST2 or Ig Isotype control staining was derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of IL-33 Receptor ST2 expression by Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat No. 553141/553142 . The splenic leukocytes were then stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 followed by staining with BD Horizon BV711 Rat Anti-Mouse CD4 antibody Cat. No. 563050 and with either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Plot or BD Horizon BB515 Rat Anti-Mouse IL-33 Receptor ST2 antibody Cat. No. 570078/570159; Right Plot at 1 mug/test. The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 563486 . The bivariate pseudocolor density plot showing the correlated expression of Foxp3 versus IL-33 Receptor ST2 or Ig Isotype control staining was derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of IL-33 Receptor ST2 expression by Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat No. 553141/553142 . The splenic leukocytes were then stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 followed by staining with BD Horizon BV711 Rat Anti-Mouse CD4 antibody Cat. No. 563050 and with either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Plot or BD Horizon BB515 Rat Anti-Mouse IL-33 Receptor ST2 antibody Cat. No. 570078/570159; Right Plot at 1 mug/test. The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 563486 . The bivariate pseudocolor density plot showing the correlated expression of Foxp3 versus IL-33 Receptor ST2 or Ig Isotype control staining was derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of IL-33 Receptor ST2 expression by Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat No. 553141/553142 . The splenic leukocytes were then stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 followed by staining with BD Horizon BV711 Rat Anti-Mouse CD4 antibody Cat. No. 563050 and with either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Plot or BD Horizon BB515 Rat Anti-Mouse IL-33 Receptor ST2 antibody Cat. No. 570078/570159; Right Plot at 1 mug/test. The cells were then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with Alexa Fluor 647 Rat Anti-Mouse Foxp3 Cat. No. 563486 . The bivariate pseudocolor density plot showing the correlated expression of Foxp3 versus IL-33 Receptor ST2 or Ig Isotype control staining was derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.