BD HorizonTM BB515 Rat Anti-Mouse MerTK (Mer)

Description

Multiparameter flow cytometric analysis of MerTK Mer expression on viable normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and with either no BD Horizon BB515 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BB515 Rat Anti-Mouse MerTK Mer antibody Cat. No. 570323/570399; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MerTK Mer or cellular Autofluorescence versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a LSR Fortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of MerTK Mer expression on viable normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and with either no BD Horizon BB515 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BB515 Rat Anti-Mouse MerTK Mer antibody Cat. No. 570323/570399; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MerTK Mer or cellular Autofluorescence versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a LSR Fortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of MerTK Mer expression on viable normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and with either no BD Horizon BB515 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BB515 Rat Anti-Mouse MerTK Mer antibody Cat. No. 570323/570399; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MerTK Mer or cellular Autofluorescence versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a LSR Fortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of MerTK Mer expression on viable normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and with either no BD Horizon BB515 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BB515 Rat Anti-Mouse MerTK Mer antibody Cat. No. 570323/570399; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MerTK Mer or cellular Autofluorescence versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a LSR Fortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of MerTK Mer expression on viable normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and with either no BD Horizon BB515 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BB515 Rat Anti-Mouse MerTK Mer antibody Cat. No. 570323/570399; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MerTK Mer or cellular Autofluorescence versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a LSR Fortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of MerTK Mer expression on viable normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and with either no BD Horizon BB515 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BB515 Rat Anti-Mouse MerTK Mer antibody Cat. No. 570323/570399; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MerTK Mer or cellular Autofluorescence versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a LSR Fortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.