BD HorizonTM BB515 Rat Anti-Mouse Siglec-F

Description

Two-color flow cytometric analysis of Siglec-F expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD11b antibody Cat. No. 553312/561690 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse Siglec-F Cat. No. 564514; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of Siglec-F or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Siglec-F expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD11b antibody Cat. No. 553312/561690 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse Siglec-F Cat. No. 564514; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of Siglec-F or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Siglec-F expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD11b antibody Cat. No. 553312/561690 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse Siglec-F Cat. No. 564514; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of Siglec-F or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Siglec-F expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD11b antibody Cat. No. 553312/561690 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse Siglec-F Cat. No. 564514; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of Siglec-F or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Siglec-F expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD11b antibody Cat. No. 553312/561690 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse Siglec-F Cat. No. 564514; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of Siglec-F or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Siglec-F expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD11b antibody Cat. No. 553312/561690 and either BD Horizon BB515 Rat IgG2a, kappa Isotype Control Cat. No. 564418; Left Panel or BD Horizon BB515 Rat Anti-Mouse Siglec-F Cat. No. 564514; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of Siglec-F or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.