BD HorizonTM BB700 Armenian Hamster Anti-Mouse CD3e

Description

Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Splenic leucocytes from a C57/BL6 mouse were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BB700 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 566421; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD3e antibody Cat. No. 566494/566495; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color dot plot showing CD3e expression or Ig Isotype Control staining versus CD4 and CD8 was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Splenic leucocytes from a C57/BL6 mouse were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BB700 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 566421; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD3e antibody Cat. No. 566494/566495; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color dot plot showing CD3e expression or Ig Isotype Control staining versus CD4 and CD8 was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Splenic leucocytes from a C57/BL6 mouse were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BB700 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 566421; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD3e antibody Cat. No. 566494/566495; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color dot plot showing CD3e expression or Ig Isotype Control staining versus CD4 and CD8 was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Splenic leucocytes from a C57/BL6 mouse were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BB700 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 566421; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD3e antibody Cat. No. 566494/566495; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color dot plot showing CD3e expression or Ig Isotype Control staining versus CD4 and CD8 was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Splenic leucocytes from a C57/BL6 mouse were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BB700 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 566421; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD3e antibody Cat. No. 566494/566495; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color dot plot showing CD3e expression or Ig Isotype Control staining versus CD4 and CD8 was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Splenic leucocytes from a C57/BL6 mouse were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies and either BD Horizon BB700 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 566421; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD3e antibody Cat. No. 566494/566495; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color dot plot showing CD3e expression or Ig Isotype Control staining versus CD4 and CD8 was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.