BD HorizonTM BB700 Armenian Hamster Anti-Mouse CD69

Description

Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours at 37C with Phorbol 12-Myristate 13-Acetate PMA, 10 ng/mL; Sigma-Aldrich, Cat. No. P-8139 . The stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Hamster IgG1, kappa Isotype Control Cat. No. 566421; dashed line histogram or BD Horizon BB700 Hamster Anti-Mouse CD69 antibody Cat. No. 566500/566501; solid line histogram at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable stimulated leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours at 37C with Phorbol 12-Myristate 13-Acetate PMA, 10 ng/mL; Sigma-Aldrich, Cat. No. P-8139 . The stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Hamster IgG1, kappa Isotype Control Cat. No. 566421; dashed line histogram or BD Horizon BB700 Hamster Anti-Mouse CD69 antibody Cat. No. 566500/566501; solid line histogram at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable stimulated leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours at 37C with Phorbol 12-Myristate 13-Acetate PMA, 10 ng/mL; Sigma-Aldrich, Cat. No. P-8139 . The stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Hamster IgG1, kappa Isotype Control Cat. No. 566421; dashed line histogram or BD Horizon BB700 Hamster Anti-Mouse CD69 antibody Cat. No. 566500/566501; solid line histogram at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable stimulated leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.