BD HorizonTM BB700 Hamster Anti-Mouse CD279 (PD-1)

Description

Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenocytes. Splenocytes from a C57BL/6 mouse were activated in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either a BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; dashed line histograms or with the BD Horizon BB700 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 562584/565942; solid line histograms at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 expression or Ig Isotype control staining were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenocytes. Splenocytes from a C57BL/6 mouse were activated in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either a BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; dashed line histograms or with the BD Horizon BB700 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 562584/565942; solid line histograms at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 expression or Ig Isotype control staining were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenocytes. Splenocytes from a C57BL/6 mouse were activated in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either a BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; dashed line histograms or with the BD Horizon BB700 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 562584/565942; solid line histograms at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 expression or Ig Isotype control staining were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenocytes. Splenocytes from a C57BL/6 mouse were activated in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either a BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; dashed line histograms or with the BD Horizon BB700 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 562584/565942; solid line histograms at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 expression or Ig Isotype control staining were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenocytes. Splenocytes from a C57BL/6 mouse were activated in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either a BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; dashed line histograms or with the BD Horizon BB700 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 562584/565942; solid line histograms at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 expression or Ig Isotype control staining were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenocytes. Splenocytes from a C57BL/6 mouse were activated in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either a BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; dashed line histograms or with the BD Horizon BB700 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 562584/565942; solid line histograms at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 expression or Ig Isotype control staining were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.