BD HorizonTM BB700 Hamster Anti-Mouse CD28

Description

Multicolor flow cytometric analysis of CD28 expression on C57BL/6 mouse splenocytes. Splenic leucocytes were pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained simultaneously with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8 Cat. No. 553030/553031/561966 antibodies and with either BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD28 antibody Cat. No. 566512/566513; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression patterns of CD28 or Ig Isotype Control staining versus CD4 and CD8 were derived for DAPI-negative gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Multicolor flow cytometric analysis of CD28 expression on C57BL/6 mouse splenocytes. Splenic leucocytes were pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained simultaneously with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8 Cat. No. 553030/553031/561966 antibodies and with either BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD28 antibody Cat. No. 566512/566513; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression patterns of CD28 or Ig Isotype Control staining versus CD4 and CD8 were derived for DAPI-negative gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Multicolor flow cytometric analysis of CD28 expression on C57BL/6 mouse splenocytes. Splenic leucocytes were pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained simultaneously with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8 Cat. No. 553030/553031/561966 antibodies and with either BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD28 antibody Cat. No. 566512/566513; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression patterns of CD28 or Ig Isotype Control staining versus CD4 and CD8 were derived for DAPI-negative gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD28 expression on C57BL/6 mouse splenocytes. Splenic leucocytes were pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained simultaneously with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8 Cat. No. 553030/553031/561966 antibodies and with either BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD28 antibody Cat. No. 566512/566513; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression patterns of CD28 or Ig Isotype Control staining versus CD4 and CD8 were derived for DAPI-negative gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD28 expression on C57BL/6 mouse splenocytes. Splenic leucocytes were pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained simultaneously with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8 Cat. No. 553030/553031/561966 antibodies and with either BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD28 antibody Cat. No. 566512/566513; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression patterns of CD28 or Ig Isotype Control staining versus CD4 and CD8 were derived for DAPI-negative gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Multicolor flow cytometric analysis of CD28 expression on C57BL/6 mouse splenocytes. Splenic leucocytes were pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained simultaneously with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8 Cat. No. 553030/553031/561966 antibodies and with either BD Horizon BB700 Hamster IgG2, kappa Isotype Control Cat. No. 566422; Left Plot or BD Horizon BB700 Hamster Anti-Mouse CD28 antibody Cat. No. 566512/566513; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression patterns of CD28 or Ig Isotype Control staining versus CD4 and CD8 were derived for DAPI-negative gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System.