BD HorizonTM BB700 Mouse Anti-GATA3

Description

Flow cytometric analysis of GATA3 expression in Ramos and Jurkat cells. Cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram and Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BB700 Mouse Anti-GATA3 antibody Cat. No. 566642/566643 at 0.5 mug/test. The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of GATA3 expression in Ramos and Jurkat cells. Cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram and Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BB700 Mouse Anti-GATA3 antibody Cat. No. 566642/566643 at 0.5 mug/test. The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of GATA3 expression in Ramos and Jurkat cells. Cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram and Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BB700 Mouse Anti-GATA3 antibody Cat. No. 566642/566643 at 0.5 mug/test. The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.