
566560 · 25 Tests
BDB Bioscience · Cat: 566560
Reach 3+ verified distributors
Manufacturer product page
bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD134 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated PHA; Sigma L-1668; 3 days peripheral blood mononuclear cells were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD134 antibody Cat. No. 566559/566560; solid line histogram . BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD134 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Flow cytometric analysis of CD134 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated PHA; Sigma L-1668; 3 days peripheral blood mononuclear cells were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD134 antibody Cat. No. 566559/566560; solid line histogram . BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD134 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Show More Flow cytometric analysis of CD134 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated PHA; Sigma L-1668; 3 days peripheral blood mononuclear cells were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD134 antibody Cat. No. 566559/566560; solid line histogram . BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD134 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System.