BD HorizonTM BB700 Mouse Anti-Human CD194

Description

Multicolor flow cytometric analysis of CD194 expression on human peripheral blood lymphocytes. Human whole blood was treated with BD PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were washed and then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842; Top Plots and BD Horizon BV421 Mouse Anti-Human CD8a Cat. No. 562429/562428; Bottom Plots antibodies, and either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; Left Plots or BD Horizon BB700 Mouse Anti-Human CD194 antibody Cat. No. 566475/566476; Right Plots . Two-color flow cytometric dot plots showing the correlated expression patterns of CD194 or Ig Isotype control staining versus CD4 or CD8a were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometr9c analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD194 expression on human peripheral blood lymphocytes. Human whole blood was treated with BD PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were washed and then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842; Top Plots and BD Horizon BV421 Mouse Anti-Human CD8a Cat. No. 562429/562428; Bottom Plots antibodies, and either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; Left Plots or BD Horizon BB700 Mouse Anti-Human CD194 antibody Cat. No. 566475/566476; Right Plots . Two-color flow cytometric dot plots showing the correlated expression patterns of CD194 or Ig Isotype control staining versus CD4 or CD8a were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometr9c analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD194 expression on human peripheral blood lymphocytes. Human whole blood was treated with BD PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were washed and then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842; Top Plots and BD Horizon BV421 Mouse Anti-Human CD8a Cat. No. 562429/562428; Bottom Plots antibodies, and either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; Left Plots or BD Horizon BB700 Mouse Anti-Human CD194 antibody Cat. No. 566475/566476; Right Plots . Two-color flow cytometric dot plots showing the correlated expression patterns of CD194 or Ig Isotype control staining versus CD4 or CD8a were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometr9c analysis was performed using a BD LSRFortessa Cell Analyzer System.