BD HorizonTM BB700 Mouse Anti-Human CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots: Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404 or BD Horizon BB700 Mouse Anti-Human CD25 antibody Cat. No. 566447/566448 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots: Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404 or BD Horizon BB700 Mouse Anti-Human CD25 antibody Cat. No. 566447/566448 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots: Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404 or BD Horizon BB700 Mouse Anti-Human CD25 antibody Cat. No. 566447/566448 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots: Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404 or BD Horizon BB700 Mouse Anti-Human CD25 antibody Cat. No. 566447/566448 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots: Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404 or BD Horizon BB700 Mouse Anti-Human CD25 antibody Cat. No. 566447/566448 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots: Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404 or BD Horizon BB700 Mouse Anti-Human CD25 antibody Cat. No. 566447/566448 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.