
566474 · 25 Tests
BDB Bioscience · Cat: 566474
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD86 expression Left Plot: Cells from the human Daudi Burkitts lymphoma, ATCC CRL-213 cell line were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD86 antibody Cat. No. 566473/566474; solid line histogram . The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable Daudi cells. Middle and Right Plots: Multiparameter flow cytometric analysis of CD86 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control or BD Horizon BB700 Mouse Anti-Human CD86 antibody. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD86 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD86 expression Left Plot: Cells from the human Daudi Burkitts lymphoma, ATCC CRL-213 cell line were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD86 antibody Cat. No. 566473/566474; solid line histogram . The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable Daudi cells. Middle and Right Plots: Multiparameter flow cytometric analysis of CD86 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control or BD Horizon BB700 Mouse Anti-Human CD86 antibody. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD86 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD86 expression Left Plot: Cells from the human Daudi Burkitts lymphoma, ATCC CRL-213 cell line were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD86 antibody Cat. No. 566473/566474; solid line histogram . The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable Daudi cells. Middle and Right Plots: Multiparameter flow cytometric analysis of CD86 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control or BD Horizon BB700 Mouse Anti-Human CD86 antibody. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD86 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD86 expression Left Plot: Cells from the human Daudi Burkitts lymphoma, ATCC CRL-213 cell line were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD86 antibody Cat. No. 566473/566474; solid line histogram . The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable Daudi cells. Middle and Right Plots: Multiparameter flow cytometric analysis of CD86 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control or BD Horizon BB700 Mouse Anti-Human CD86 antibody. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD86 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD86 expression Left Plot: Cells from the human Daudi Burkitts lymphoma, ATCC CRL-213 cell line were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD86 antibody Cat. No. 566473/566474; solid line histogram . The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable Daudi cells. Middle and Right Plots: Multiparameter flow cytometric analysis of CD86 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control or BD Horizon BB700 Mouse Anti-Human CD86 antibody. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD86 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD86 expression Left Plot: Cells from the human Daudi Burkitts lymphoma, ATCC CRL-213 cell line were stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; dashed line histogram or BD Horizon BB700 Mouse Anti-Human CD86 antibody Cat. No. 566473/566474; solid line histogram . The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable Daudi cells. Middle and Right Plots: Multiparameter flow cytometric analysis of CD86 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control or BD Horizon BB700 Mouse Anti-Human CD86 antibody. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric dot plots showing the correlated expression of CD86 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.