BD HorizonTM BB700 Mouse Anti-Human FoxP3

Description

Multicolor flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells were stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 and BD Horizon BV421 Anti-Human CD25 Cat. No. 562442/562443 antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BB700 Mouse Anti-Human FoxP3 antibody Cat No. 566526/566527 . Two-color flow cytometric dot plots showing the correlated expression of either FoxP3 versus CD25 Left Plot or CD4 versus FoxP3 Right Plot were derived from gated events with the light scattering characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells were stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 and BD Horizon BV421 Anti-Human CD25 Cat. No. 562442/562443 antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BB700 Mouse Anti-Human FoxP3 antibody Cat No. 566526/566527 . Two-color flow cytometric dot plots showing the correlated expression of either FoxP3 versus CD25 Left Plot or CD4 versus FoxP3 Right Plot were derived from gated events with the light scattering characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells were stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 and BD Horizon BV421 Anti-Human CD25 Cat. No. 562442/562443 antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BB700 Mouse Anti-Human FoxP3 antibody Cat No. 566526/566527 . Two-color flow cytometric dot plots showing the correlated expression of either FoxP3 versus CD25 Left Plot or CD4 versus FoxP3 Right Plot were derived from gated events with the light scattering characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells were stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 and BD Horizon BV421 Anti-Human CD25 Cat. No. 562442/562443 antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BB700 Mouse Anti-Human FoxP3 antibody Cat No. 566526/566527 . Two-color flow cytometric dot plots showing the correlated expression of either FoxP3 versus CD25 Left Plot or CD4 versus FoxP3 Right Plot were derived from gated events with the light scattering characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells were stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 and BD Horizon BV421 Anti-Human CD25 Cat. No. 562442/562443 antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BB700 Mouse Anti-Human FoxP3 antibody Cat No. 566526/566527 . Two-color flow cytometric dot plots showing the correlated expression of either FoxP3 versus CD25 Left Plot or CD4 versus FoxP3 Right Plot were derived from gated events with the light scattering characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells were stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 and BD Horizon BV421 Anti-Human CD25 Cat. No. 562442/562443 antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BB700 Mouse Anti-Human FoxP3 antibody Cat No. 566526/566527 . Two-color flow cytometric dot plots showing the correlated expression of either FoxP3 versus CD25 Left Plot or CD4 versus FoxP3 Right Plot were derived from gated events with the light scattering characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.