BD HorizonTM BB700 Mouse Anti-Human IL-22

Description

Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate PMA; Sigma, 50 ng/ml and Ionomycin Sigma, 500 ng/ml in the presence of BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424/562425 and either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; Left Plot or BD Horizon BB700 Mouse anti-Human IL-22 antibody Cat. No. 567688/567689; Right Plot at 0.125 mug/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate PMA; Sigma, 50 ng/ml and Ionomycin Sigma, 500 ng/ml in the presence of BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424/562425 and either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; Left Plot or BD Horizon BB700 Mouse anti-Human IL-22 antibody Cat. No. 567688/567689; Right Plot at 0.125 mug/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate PMA; Sigma, 50 ng/ml and Ionomycin Sigma, 500 ng/ml in the presence of BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424/562425 and either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; Left Plot or BD Horizon BB700 Mouse anti-Human IL-22 antibody Cat. No. 567688/567689; Right Plot at 0.125 mug/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate PMA; Sigma, 50 ng/ml and Ionomycin Sigma, 500 ng/ml in the presence of BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424/562425 and either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; Left Plot or BD Horizon BB700 Mouse anti-Human IL-22 antibody Cat. No. 567688/567689; Right Plot at 0.125 mug/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate PMA; Sigma, 50 ng/ml and Ionomycin Sigma, 500 ng/ml in the presence of BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424/562425 and either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; Left Plot or BD Horizon BB700 Mouse anti-Human IL-22 antibody Cat. No. 567688/567689; Right Plot at 0.125 mug/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate PMA; Sigma, 50 ng/ml and Ionomycin Sigma, 500 ng/ml in the presence of BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424/562425 and either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; Left Plot or BD Horizon BB700 Mouse anti-Human IL-22 antibody Cat. No. 567688/567689; Right Plot at 0.125 mug/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.