BD HorizonTM BB700 Mouse Anti-Human TIGIT

Description

Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leukocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 Cat. No. 555516; Middle Plots , BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , and APC Mouse Anti-Human CD45RO Cat. No. 559865; Right Plots antibodies, and with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; Top Plots or BD Horizon BB700 Mouse Anti-Human TIGIT antibody Cat. No. 570307/570313; Bottom Plots as indicated. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Left Plots - The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leukocyte populations. Middle Plots - The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plots - The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the forward and side light-scatter characteristics of viable lymphocytes. Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leukocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 Cat. No. 555516; Middle Plots , BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , and APC Mouse Anti-Human CD45RO Cat. No. 559865; Right Plots antibodies, and with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; Top Plots or BD Horizon BB700 Mouse Anti-Human TIGIT antibody Cat. No. 570307/570313; Bottom Plots as indicated. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Left Plots - The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leukocyte populations. Middle Plots - The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plots - The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leukocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 Cat. No. 555516; Middle Plots , BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , and APC Mouse Anti-Human CD45RO Cat. No. 559865; Right Plots antibodies, and with either BD Horizon BB700 Mouse IgG1, kappa Isotype Control Cat. No. 566404; Top Plots or BD Horizon BB700 Mouse Anti-Human TIGIT antibody Cat. No. 570307/570313; Bottom Plots as indicated. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Left Plots - The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leukocyte populations. Middle Plots - The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plots - The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the forward and side light-scatter characteristics of viable lymphocytes.