BD HorizonTM BB700 Mouse Anti-Mouse CD45.2

Description

Flow cytometric analysis of CD45.2 expression on C57BL/6 mouse splenocytes. Splenic leucocytes from an SJL mouse Left Plot or a C57BL/6 mouse Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; dashed line histograms or BD Horizon BB700 Mouse Anti-Mouse CD45.2 antibody Cat. No 566496/566497; solid line histogram at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45.2 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.2 expression on C57BL/6 mouse splenocytes. Splenic leucocytes from an SJL mouse Left Plot or a C57BL/6 mouse Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; dashed line histograms or BD Horizon BB700 Mouse Anti-Mouse CD45.2 antibody Cat. No 566496/566497; solid line histogram at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45.2 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.2 expression on C57BL/6 mouse splenocytes. Splenic leucocytes from an SJL mouse Left Plot or a C57BL/6 mouse Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; dashed line histograms or BD Horizon BB700 Mouse Anti-Mouse CD45.2 antibody Cat. No 566496/566497; solid line histogram at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45.2 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45.2 expression on C57BL/6 mouse splenocytes. Splenic leucocytes from an SJL mouse Left Plot or a C57BL/6 mouse Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; dashed line histograms or BD Horizon BB700 Mouse Anti-Mouse CD45.2 antibody Cat. No 566496/566497; solid line histogram at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45.2 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45.2 expression on C57BL/6 mouse splenocytes. Splenic leucocytes from an SJL mouse Left Plot or a C57BL/6 mouse Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; dashed line histograms or BD Horizon BB700 Mouse Anti-Mouse CD45.2 antibody Cat. No 566496/566497; solid line histogram at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45.2 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.2 expression on C57BL/6 mouse splenocytes. Splenic leucocytes from an SJL mouse Left Plot or a C57BL/6 mouse Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419; dashed line histograms or BD Horizon BB700 Mouse Anti-Mouse CD45.2 antibody Cat. No 566496/566497; solid line histogram at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45.2 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.