BD HorizonTM BB700 Mouse Anti-Mouse NK-1.1

Description

Two-color flow cytometric analysis of NK-1.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062/553061/561827 and either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419, Left Plot or BD Horizon BB700 Mouse Anti-Mouse NK-1.1 Cat. No. 566502/566503; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of NK-1.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062/553061/561827 and either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419, Left Plot or BD Horizon BB700 Mouse Anti-Mouse NK-1.1 Cat. No. 566502/566503; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of NK-1.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062/553061/561827 and either BD Horizon BB700 Mouse IgG2a, kappa Isotype Control Cat. No. 566419, Left Plot or BD Horizon BB700 Mouse Anti-Mouse NK-1.1 Cat. No. 566502/566503; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.