

566438 · 25 Tests
BDB Bioscience · Cat: 566438
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Description
Multicolor flow cytometric analysis of CD197 CCR7 and CD45RA coexpression patterns on CD4+ and CD8+ human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 , BD Horizon BB700 Rat Anti-Human CD197 CCR7 Cat. No. 566437/566438 , FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , and APC Mouse Anti-Human CD8 Cat. No. 555369/561421/561952/561953 antibodies. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plots showing the correlated expression of CD45RA versus CD197 CCR7 were derived for CD4+ Left Plot or CD8+ Right Plot gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Multicolor flow cytometric analysis of CD197 CCR7 and CD45RA coexpression patterns on CD4+ and CD8+ human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 , BD Horizon BB700 Rat Anti-Human CD197 CCR7 Cat. No. 566437/566438 , FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , and APC Mouse Anti-Human CD8 Cat. No. 555369/561421/561952/561953 antibodies. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plots showing the correlated expression of CD45RA versus CD197 CCR7 were derived for CD4+ Left Plot or CD8+ Right Plot gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Multicolor flow cytometric analysis of CD197 CCR7 and CD45RA coexpression patterns on CD4+ and CD8+ human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 , BD Horizon BB700 Rat Anti-Human CD197 CCR7 Cat. No. 566437/566438 , FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , and APC Mouse Anti-Human CD8 Cat. No. 555369/561421/561952/561953 antibodies. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plots showing the correlated expression of CD45RA versus CD197 CCR7 were derived for CD4+ Left Plot or CD8+ Right Plot gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD197 CCR7 and CD45RA coexpression patterns on CD4+ and CD8+ human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 , BD Horizon BB700 Rat Anti-Human CD197 CCR7 Cat. No. 566437/566438 , FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , and APC Mouse Anti-Human CD8 Cat. No. 555369/561421/561952/561953 antibodies. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plots showing the correlated expression of CD45RA versus CD197 CCR7 were derived for CD4+ Left Plot or CD8+ Right Plot gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD197 CCR7 and CD45RA coexpression patterns on CD4+ and CD8+ human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 , BD Horizon BB700 Rat Anti-Human CD197 CCR7 Cat. No. 566437/566438 , FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , and APC Mouse Anti-Human CD8 Cat. No. 555369/561421/561952/561953 antibodies. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plots showing the correlated expression of CD45RA versus CD197 CCR7 were derived for CD4+ Left Plot or CD8+ Right Plot gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Multicolor flow cytometric analysis of CD197 CCR7 and CD45RA coexpression patterns on CD4+ and CD8+ human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 , BD Horizon BB700 Rat Anti-Human CD197 CCR7 Cat. No. 566437/566438 , FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , and APC Mouse Anti-Human CD8 Cat. No. 555369/561421/561952/561953 antibodies. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plots showing the correlated expression of CD45RA versus CD197 CCR7 were derived for CD4+ Left Plot or CD8+ Right Plot gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System.