BD HorizonTM BB700 Rat Anti-Human IL-2

Description

Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat No. 562426/562427 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Human IL-2 antibody Cat No. 566405/566406; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat No. 562426/562427 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Human IL-2 antibody Cat No. 566405/566406; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat No. 562426/562427 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Human IL-2 antibody Cat No. 566405/566406; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat No. 562426/562427 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Human IL-2 antibody Cat No. 566405/566406; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat No. 562426/562427 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Human IL-2 antibody Cat No. 566405/566406; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat No. 562426/562427 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Human IL-2 antibody Cat No. 566405/566406; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System.