BD HorizonTM BB700 Rat Anti-Mouse CD197 (CCR7)

Description

Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD197 CCR7 antibody Cat. No. 566462/566464; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color flow cytometric dot plot showing the correlated expression of CD197 CCR7 or Ig Isotype control staining versus CD3e was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD197 CCR7 antibody Cat. No. 566462/566464; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color flow cytometric dot plot showing the correlated expression of CD197 CCR7 or Ig Isotype control staining versus CD3e was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD197 CCR7 antibody Cat. No. 566462/566464; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color flow cytometric dot plot showing the correlated expression of CD197 CCR7 or Ig Isotype control staining versus CD3e was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD197 CCR7 antibody Cat. No. 566462/566464; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color flow cytometric dot plot showing the correlated expression of CD197 CCR7 or Ig Isotype control staining versus CD3e was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD197 CCR7 antibody Cat. No. 566462/566464; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color flow cytometric dot plot showing the correlated expression of CD197 CCR7 or Ig Isotype control staining versus CD3e was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD197 CCR7 antibody Cat. No. 566462/566464; Right Plot at 1 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The two-color flow cytometric dot plot showing the correlated expression of CD197 CCR7 or Ig Isotype control staining versus CD3e was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.