BD HorizonTM BB700 Rat Anti-Mouse CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenic leucocytes. Left and Middle Plots : Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BB700 Rat IgG1, kappa Isotype Control Cat. No. 566420; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD25 antibody Cat. No. 566498/566499; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot : Mouse splenic leucocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon BB700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB700 Rat Anti-Mouse CD25 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from DAPI negative-events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenic leucocytes. Left and Middle Plots : Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BB700 Rat IgG1, kappa Isotype Control Cat. No. 566420; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD25 antibody Cat. No. 566498/566499; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot : Mouse splenic leucocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon BB700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB700 Rat Anti-Mouse CD25 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from DAPI negative-events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenic leucocytes. Left and Middle Plots : Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BB700 Rat IgG1, kappa Isotype Control Cat. No. 566420; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD25 antibody Cat. No. 566498/566499; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot : Mouse splenic leucocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon BB700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB700 Rat Anti-Mouse CD25 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from DAPI negative-events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenic leucocytes. Left and Middle Plots : Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BB700 Rat IgG1, kappa Isotype Control Cat. No. 566420; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD25 antibody Cat. No. 566498/566499; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot : Mouse splenic leucocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon BB700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB700 Rat Anti-Mouse CD25 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from DAPI negative-events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenic leucocytes. Left and Middle Plots : Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BB700 Rat IgG1, kappa Isotype Control Cat. No. 566420; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD25 antibody Cat. No. 566498/566499; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot : Mouse splenic leucocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon BB700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB700 Rat Anti-Mouse CD25 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from DAPI negative-events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenic leucocytes. Left and Middle Plots : Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BB700 Rat IgG1, kappa Isotype Control Cat. No. 566420; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD25 antibody Cat. No. 566498/566499; Right Plot at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot : Mouse splenic leucocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon BB700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB700 Rat Anti-Mouse CD25 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from DAPI negative-events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.