BD HorizonTM BB700 Rat Anti-Mouse CD31

Description

Two-color flow cytometric analysis of CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD31 antibody Cat. No. 566490/566491; Right Plot at 0.125 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD31 antibody Cat. No. 566490/566491; Right Plot at 0.125 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD31 antibody Cat. No. 566490/566491; Right Plot at 0.125 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD31 antibody Cat. No. 566490/566491; Right Plot at 0.125 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD31 antibody Cat. No. 566490/566491; Right Plot at 0.125 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD31 antibody Cat. No. 566490/566491; Right Plot at 0.125 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.