BD HorizonTM BB700 Rat Anti-Mouse CD4

Description

Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD4 antibody Cat. No. 566407/566408; Right Plot at 0.5 mug/test. The two-color contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSymphony A5 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD4 antibody Cat. No. 566407/566408; Right Plot at 0.5 mug/test. The two-color contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSymphony A5 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD4 antibody Cat. No. 566407/566408; Right Plot at 0.5 mug/test. The two-color contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSymphony A5 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD4 antibody Cat. No. 566407/566408; Right Plot at 0.5 mug/test. The two-color contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSymphony A5 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD4 antibody Cat. No. 566407/566408; Right Plot at 0.5 mug/test. The two-color contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSymphony A5 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; Left Plot or BD Horizon BB700 Rat Anti-Mouse CD4 antibody Cat. No. 566407/566408; Right Plot at 0.5 mug/test. The two-color contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD FACSymphony A5 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.