BD HorizonTM BB700 Rat Anti-Mouse CD43

Description

Flow cytometric analysis of CD43 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; dashed line histogram or BD Horizon BB700 Rat Anti-Mouse CD43 antibody Cat. No. 566508/566509; solid line histogram at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD43 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD43 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; dashed line histogram or BD Horizon BB700 Rat Anti-Mouse CD43 antibody Cat. No. 566508/566509; solid line histogram at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD43 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD43 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; dashed line histogram or BD Horizon BB700 Rat Anti-Mouse CD43 antibody Cat. No. 566508/566509; solid line histogram at 0.5 mug/test. BD Pharmingen DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD43 expression or Ig Isotype control staining was derived from DAPI negative-gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD FACSCelesta Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.