BD HorizonTM BB700 Rat Anti-Mouse CD49b

Description

Multicolor flow cytometric analysis of CD49b expressed on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Mouse Anti-Mouse NK1.1 antibody Cat. No. 564144 and were either not stained Autofluorescence Control; Left Plot or stained with BD Horizon BB700 Rat Anti-Mouse CD49b antibody Cat. No. 568015, Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD49b or Autofluorescence versus NK1.1 expression was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD49b expressed on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Mouse Anti-Mouse NK1.1 antibody Cat. No. 564144 and were either not stained Autofluorescence Control; Left Plot or stained with BD Horizon BB700 Rat Anti-Mouse CD49b antibody Cat. No. 568015, Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD49b or Autofluorescence versus NK1.1 expression was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD49b expressed on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Mouse Anti-Mouse NK1.1 antibody Cat. No. 564144 and were either not stained Autofluorescence Control; Left Plot or stained with BD Horizon BB700 Rat Anti-Mouse CD49b antibody Cat. No. 568015, Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD49b or Autofluorescence versus NK1.1 expression was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD49b expressed on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Mouse Anti-Mouse NK1.1 antibody Cat. No. 564144 and were either not stained Autofluorescence Control; Left Plot or stained with BD Horizon BB700 Rat Anti-Mouse CD49b antibody Cat. No. 568015, Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD49b or Autofluorescence versus NK1.1 expression was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD49b expressed on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Mouse Anti-Mouse NK1.1 antibody Cat. No. 564144 and were either not stained Autofluorescence Control; Left Plot or stained with BD Horizon BB700 Rat Anti-Mouse CD49b antibody Cat. No. 568015, Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD49b or Autofluorescence versus NK1.1 expression was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD49b expressed on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Mouse Anti-Mouse NK1.1 antibody Cat. No. 564144 and were either not stained Autofluorescence Control; Left Plot or stained with BD Horizon BB700 Rat Anti-Mouse CD49b antibody Cat. No. 568015, Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD49b or Autofluorescence versus NK1.1 expression was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.