BD HorizonTM BB700 Rat Anti-Mouse Ly-6G

Description

Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; dashed line histogram or BD Horizon BB700 Rat Anti-Mouse LY-6G antibody Cat. No. 566435/566453; solid line histogram at 0.5 mug/ml. Cells were then stained with BD Via-Probe Red Nucleic Acid Stain Cat. No. 565803/565804 and analyzed. The fluorescence histograms showing Ly-6G fluorescence or Ig Isotype control staining were derived from gated events with the light scattering characteristics of viable lymphoid Left Panel or myeloid Right Panel cells that were Via Probe Red-negative. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; dashed line histogram or BD Horizon BB700 Rat Anti-Mouse LY-6G antibody Cat. No. 566435/566453; solid line histogram at 0.5 mug/ml. Cells were then stained with BD Via-Probe Red Nucleic Acid Stain Cat. No. 565803/565804 and analyzed. The fluorescence histograms showing Ly-6G fluorescence or Ig Isotype control staining were derived from gated events with the light scattering characteristics of viable lymphoid Left Panel or myeloid Right Panel cells that were Via Probe Red-negative. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; dashed line histogram or BD Horizon BB700 Rat Anti-Mouse LY-6G antibody Cat. No. 566435/566453; solid line histogram at 0.5 mug/ml. Cells were then stained with BD Via-Probe Red Nucleic Acid Stain Cat. No. 565803/565804 and analyzed. The fluorescence histograms showing Ly-6G fluorescence or Ig Isotype control staining were derived from gated events with the light scattering characteristics of viable lymphoid Left Panel or myeloid Right Panel cells that were Via Probe Red-negative. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; dashed line histogram or BD Horizon BB700 Rat Anti-Mouse LY-6G antibody Cat. No. 566435/566453; solid line histogram at 0.5 mug/ml. Cells were then stained with BD Via-Probe Red Nucleic Acid Stain Cat. No. 565803/565804 and analyzed. The fluorescence histograms showing Ly-6G fluorescence or Ig Isotype control staining were derived from gated events with the light scattering characteristics of viable lymphoid Left Panel or myeloid Right Panel cells that were Via Probe Red-negative. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; dashed line histogram or BD Horizon BB700 Rat Anti-Mouse LY-6G antibody Cat. No. 566435/566453; solid line histogram at 0.5 mug/ml. Cells were then stained with BD Via-Probe Red Nucleic Acid Stain Cat. No. 565803/565804 and analyzed. The fluorescence histograms showing Ly-6G fluorescence or Ig Isotype control staining were derived from gated events with the light scattering characteristics of viable lymphoid Left Panel or myeloid Right Panel cells that were Via Probe Red-negative. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BB700 Rat IgG2a, kappa Isotype Control Cat. No. 566413; dashed line histogram or BD Horizon BB700 Rat Anti-Mouse LY-6G antibody Cat. No. 566435/566453; solid line histogram at 0.5 mug/ml. Cells were then stained with BD Via-Probe Red Nucleic Acid Stain Cat. No. 565803/565804 and analyzed. The fluorescence histograms showing Ly-6G fluorescence or Ig Isotype control staining were derived from gated events with the light scattering characteristics of viable lymphoid Left Panel or myeloid Right Panel cells that were Via Probe Red-negative. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.