BD HorizonTM Brilliant Stain Buffer

Description

Figure 1. BD Horizon Brilliant Stain Buffer should be used in multicolor immunofluorescent staining and flow cytometric analysis when using two or more BD Horizon Brilliant reagents. Whole human blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody and either BD Horizon BV605 Mouse Anti-Human CD8 antibody Left Panels or BD Horizon BV786 Mouse Anti-Human CD19 antibody Right Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus either CD8 or CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Staining cells in the presence of BD Horizon Brilliant Stain Buffer Cat. No. 563794/566349; Lower Panels restores the major lymphocyte populations to their expected fluorescent staining patterns dotted lines when compared with cells stained without Brilliant Stain Buffer Upper Panels . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Figure 1. BD Horizon Brilliant Stain Buffer should be used in multicolor immunofluorescent staining and flow cytometric analysis when using two or more BD Horizon Brilliant reagents. Whole human blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody and either BD Horizon BV605 Mouse Anti-Human CD8 antibody Left Panels or BD Horizon BV786 Mouse Anti-Human CD19 antibody Right Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus either CD8 or CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Staining cells in the presence of BD Horizon Brilliant Stain Buffer Cat. No. 563794/566349; Lower Panels restores the major lymphocyte populations to their expected fluorescent staining patterns dotted lines when compared with cells stained without Brilliant Stain Buffer Upper Panels . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Figure 1. BD Horizon Brilliant Stain Buffer should be used in multicolor immunofluorescent staining and flow cytometric analysis when using two or more BD Horizon Brilliant reagents. Whole human blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody and either BD Horizon BV605 Mouse Anti-Human CD8 antibody Left Panels or BD Horizon BV786 Mouse Anti-Human CD19 antibody Right Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus either CD8 or CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Staining cells in the presence of BD Horizon Brilliant Stain Buffer Cat. No. 563794/566349; Lower Panels restores the major lymphocyte populations to their expected fluorescent staining patterns dotted lines when compared with cells stained without Brilliant Stain Buffer Upper Panels . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.