BD HorizonTM BUV395 Hamster Anti-Mouse TCR beta Chain

Description

Two-color flow cytometric analysis of TCR beta expression on Mouse splenocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553049/553048/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and with either BD Horizon BUV395 Hamster IgG2, kappa Isotype Control Cat. No. 565820; Left Plot or BD Horizon BUV395 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 569248; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR beta or Isotype control staining versus CD4 + CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR beta expression on Mouse splenocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553049/553048/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and with either BD Horizon BUV395 Hamster IgG2, kappa Isotype Control Cat. No. 565820; Left Plot or BD Horizon BUV395 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 569248; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR beta or Isotype control staining versus CD4 + CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR beta expression on Mouse splenocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553049/553048/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and with either BD Horizon BUV395 Hamster IgG2, kappa Isotype Control Cat. No. 565820; Left Plot or BD Horizon BUV395 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 569248; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR beta or Isotype control staining versus CD4 + CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TCR beta expression on Mouse splenocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553049/553048/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and with either BD Horizon BUV395 Hamster IgG2, kappa Isotype Control Cat. No. 565820; Left Plot or BD Horizon BUV395 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 569248; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR beta or Isotype control staining versus CD4 + CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TCR beta expression on Mouse splenocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553049/553048/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and with either BD Horizon BUV395 Hamster IgG2, kappa Isotype Control Cat. No. 565820; Left Plot or BD Horizon BUV395 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 569248; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR beta or Isotype control staining versus CD4 + CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR beta expression on Mouse splenocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553049/553048/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and with either BD Horizon BUV395 Hamster IgG2, kappa Isotype Control Cat. No. 565820; Left Plot or BD Horizon BUV395 Hamster Anti-Mouse TCR beta Chain antibody Cat. No. 569248; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR beta or Isotype control staining versus CD4 + CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.