BD HorizonTM BUV395 Mouse Anti-Biotin

Description

Multiparameter flow cytometric analysis of CD25 expression on viable Mouse splenic lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block and stained with Biotin Rat Anti-Mouse CD25 antibody Cat. No. 567831 . The cells were washed and then secondarily stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Biotin antibody Cat. No. 570939/571011; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD25 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD19 expression on viable Mouse splenic lymphocytes. Left Panel - One-layer Staining: Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; dashed line histogram or BD Horizon BUV395 Rat Anti-Mouse CD19 antibody Cat. No. 563557; solid line histogram . Right Panel - Two-layer Staining: Mouse splenic leukocytes were preincubated with Mouse BD Fc Block and then stained with Biotin Rat Anti-Mouse CD19 antibody Cat. No. 553784 , washed, and secondarily stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; dashed line histogram or BD Horizon BUV395 Mouse Anti-Biotin antibody Cat. No. 570939/571011; solid histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histograms showing CD19 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 expression on viable Mouse splenic lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block and stained with Biotin Rat Anti-Mouse CD25 antibody Cat. No. 567831 . The cells were washed and then secondarily stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Biotin antibody Cat. No. 570939/571011; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD25 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD19 expression on viable Mouse splenic lymphocytes. Left Panel - One-layer Staining: Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; dashed line histogram or BD Horizon BUV395 Rat Anti-Mouse CD19 antibody Cat. No. 563557; solid line histogram . Right Panel - Two-layer Staining: Mouse splenic leukocytes were preincubated with Mouse BD Fc Block and then stained with Biotin Rat Anti-Mouse CD19 antibody Cat. No. 553784 , washed, and secondarily stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; dashed line histogram or BD Horizon BUV395 Mouse Anti-Biotin antibody Cat. No. 570939/571011; solid histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histograms showing CD19 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 expression on viable Mouse splenic lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block and stained with Biotin Rat Anti-Mouse CD25 antibody Cat. No. 567831 . The cells were washed and then secondarily stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Biotin antibody Cat. No. 570939/571011; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD25 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD19 expression on viable Mouse splenic lymphocytes. Left Panel - One-layer Staining: Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; dashed line histogram or BD Horizon BUV395 Rat Anti-Mouse CD19 antibody Cat. No. 563557; solid line histogram . Right Panel - Two-layer Staining: Mouse splenic leukocytes were preincubated with Mouse BD Fc Block and then stained with Biotin Rat Anti-Mouse CD19 antibody Cat. No. 553784 , washed, and secondarily stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; dashed line histogram or BD Horizon BUV395 Mouse Anti-Biotin antibody Cat. No. 570939/571011; solid histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histograms showing CD19 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.