BD HorizonTM BUV395 Mouse Anti-Human CCR7 (CD197)

Description

Multiparameter flow cytometric analysis of CCR7 CD197 on Human peripheral blood leucocytes and lymphocytes. Human whole blood was stained with BD Horizon R718 Mouse Anti-Human CD4 Cat. No. 567092/567234 , BD Horizon BV421 Mouse Anti-Human CD8 Cat. No. 562428/562429 , PE Mouse Anti-Human CD45RA Cat. No. 555489/561883 antibodies, and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547 or BD Horizon BUV395 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 569108 at 2.0 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa X20 Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Plots 1 and 2: Bivariate pseudocolor density plots showing the correlated expression of CCR7 CD197 or Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Plot 3: The two-parameter pseudocolor density plot showing the correlated expression of CCR7 CD197 versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Multiparameter flow cytometric analysis of CCR7 CD197 on Human peripheral blood leucocytes and lymphocytes. Human whole blood was stained with BD Horizon R718 Mouse Anti-Human CD4 Cat. No. 567092/567234 , BD Horizon BV421 Mouse Anti-Human CD8 Cat. No. 562428/562429 , PE Mouse Anti-Human CD45RA Cat. No. 555489/561883 antibodies, and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547 or BD Horizon BUV395 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 569108 at 2.0 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa X20 Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Plots 1 and 2: Bivariate pseudocolor density plots showing the correlated expression of CCR7 CD197 or Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Plot 3: The two-parameter pseudocolor density plot showing the correlated expression of CCR7 CD197 versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Show More Multiparameter flow cytometric analysis of CCR7 CD197 on Human peripheral blood leucocytes and lymphocytes. Human whole blood was stained with BD Horizon R718 Mouse Anti-Human CD4 Cat. No. 567092/567234 , BD Horizon BV421 Mouse Anti-Human CD8 Cat. No. 562428/562429 , PE Mouse Anti-Human CD45RA Cat. No. 555489/561883 antibodies, and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547 or BD Horizon BUV395 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 569108 at 2.0 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa X20 Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Plots 1 and 2: Bivariate pseudocolor density plots showing the correlated expression of CCR7 CD197 or Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Plot 3: The two-parameter pseudocolor density plot showing the correlated expression of CCR7 CD197 versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes.