BD HorizonTM BUV395 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody Cat. No. 561844/555347/561843 and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescent histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody Cat. No. 561844/555347/561843 and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescent histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody Cat. No. 561844/555347/561843 and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescent histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody Cat. No. 561844/555347/561843 and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescent histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody Cat. No. 561844/555347/561843 and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescent histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody Cat. No. 561844/555347/561843 and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat. No. 563547; dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569353; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescent histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.