BD HorizonTM BUV395 Mouse Anti-Human CD39

Description

Flow cytometric analysis of CD39 expression on human peripheral blood leucocyte populations. Upper Plots: Whole blood was stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human CD39 antibody Cat No. 567508/567507; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD39 or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Lower Plots: Human peripheral blood mononuclear cells PBMC were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , APC Mouse Anti-Human CD25 Cat. No. 555434/560987/561399 , BD Horizon BUV737 Mouse Anti-Human CD127 Cat No. 612794/612795 antibodies, and either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD39 antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the coexpressed levels of CD25 versus CD127 by viable 7-AAD-negative light scatter-gated CD4+ T cells Left Plot was further gated to reveal CD39 expression or Ig Isotype control staining Right Plot on CD4+CD25+CD127low T cells ie, cells with a Regulatory T cell immunophenotype as shown. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Flow cytometric analysis of CD39 expression on human peripheral blood leucocyte populations. Upper Plots: Whole blood was stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human CD39 antibody Cat No. 567508/567507; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD39 or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Lower Plots: Human peripheral blood mononuclear cells PBMC were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , APC Mouse Anti-Human CD25 Cat. No. 555434/560987/561399 , BD Horizon BUV737 Mouse Anti-Human CD127 Cat No. 612794/612795 antibodies, and either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD39 antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the coexpressed levels of CD25 versus CD127 by viable 7-AAD-negative light scatter-gated CD4+ T cells Left Plot was further gated to reveal CD39 expression or Ig Isotype control staining Right Plot on CD4+CD25+CD127low T cells ie, cells with a Regulatory T cell immunophenotype as shown. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Show More Flow cytometric analysis of CD39 expression on human peripheral blood leucocyte populations. Upper Plots: Whole blood was stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human CD39 antibody Cat No. 567508/567507; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD39 or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Lower Plots: Human peripheral blood mononuclear cells PBMC were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , APC Mouse Anti-Human CD25 Cat. No. 555434/560987/561399 , BD Horizon BUV737 Mouse Anti-Human CD127 Cat No. 612794/612795 antibodies, and either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD39 antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the coexpressed levels of CD25 versus CD127 by viable 7-AAD-negative light scatter-gated CD4+ T cells Left Plot was further gated to reveal CD39 expression or Ig Isotype control staining Right Plot on CD4+CD25+CD127low T cells ie, cells with a Regulatory T cell immunophenotype as shown. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software.