

568523 ·
BDB Bioscience · Cat: 568523
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD71 expression on stimulated Human peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BUV395 Mouse IgG2a, kappa Isotype Control Cat. No. 563809; dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD71 antibody Cat. No. 568523; solid line histogram at 0.06 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD71 expression or Ig Isotype control staining was derived from events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet is not lot specific. Flow cytometric analysis of CD71 expression on stimulated Human peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BUV395 Mouse IgG2a, kappa Isotype Control Cat. No. 563809; dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD71 antibody Cat. No. 568523; solid line histogram at 0.06 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD71 expression or Ig Isotype control staining was derived from events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet is not lot specific. Show More Flow cytometric analysis of CD71 expression on stimulated Human peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BUV395 Mouse IgG2a, kappa Isotype Control Cat. No. 563809; dashed line histogram or BD Horizon BUV395 Mouse Anti-Human CD71 antibody Cat. No. 568523; solid line histogram at 0.06 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD71 expression or Ig Isotype control staining was derived from events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet is not lot specific.