BD HorizonTM BUV395 Rabbit Anti- Active Caspase-3

Description

Flow cytometric analysis of Active Caspase-3 expressed by apoptotic Jurkat cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were untreated dashed line histogram or treated solid line histogram with 4 muM camptothecin for 4 hr to induce apoptosis. The cells were washed once in Dulbeccos PBS, fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714; 20 min at room RT , pelleted and washed with BD Perm/Wash Buffer a component of the kit . The cells were subsequently stained with the BD Horizon BUV395 Rabbit Anti-Active Caspase-3 antibody Cat. No. 564095 . The cells were then washed, resuspended in BD Perm/Wash Buffer, and analyzed using a BD LSR II Flow Cytometer System. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometric analysis of Active Caspase-3 expressed by apoptotic Jurkat cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were untreated dashed line histogram or treated solid line histogram with 4 muM camptothecin for 4 hr to induce apoptosis. The cells were washed once in Dulbeccos PBS, fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714; 20 min at room RT , pelleted and washed with BD Perm/Wash Buffer a component of the kit . The cells were subsequently stained with the BD Horizon BUV395 Rabbit Anti-Active Caspase-3 antibody Cat. No. 564095 . The cells were then washed, resuspended in BD Perm/Wash Buffer, and analyzed using a BD LSR II Flow Cytometer System. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Show More Flow cytometric analysis of Active Caspase-3 expressed by apoptotic Jurkat cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were untreated dashed line histogram or treated solid line histogram with 4 muM camptothecin for 4 hr to induce apoptosis. The cells were washed once in Dulbeccos PBS, fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714; 20 min at room RT , pelleted and washed with BD Perm/Wash Buffer a component of the kit . The cells were subsequently stained with the BD Horizon BUV395 Rabbit Anti-Active Caspase-3 antibody Cat. No. 564095 . The cells were then washed, resuspended in BD Perm/Wash Buffer, and analyzed using a BD LSR II Flow Cytometer System. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells.