BD HorizonTM BUV395 Rat Anti-Mouse Blimp-1

Description

Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567832; Right Plot . Bivariate pseudocolor density plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567832; Right Plot . Bivariate pseudocolor density plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567832; Right Plot . Bivariate pseudocolor density plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567832; Right Plot . Bivariate pseudocolor density plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567832; Right Plot . Bivariate pseudocolor density plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567832; Right Plot . Bivariate pseudocolor density plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.