BD HorizonTM BUV395 Rat Anti-Mouse CD273

Description

Two-color flow cytometric analysis of CD273 expression on mouse splenic leucocytes dendritic cells . Mouse splenic leucocytes were cultured overnight with recombinant mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Panel or BD Horizon BUV395 Rat Anti-Mouse CD273 antibody Cat. No. 565102; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD273 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of CD273 expression on mouse splenic leucocytes dendritic cells . Mouse splenic leucocytes were cultured overnight with recombinant mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Panel or BD Horizon BUV395 Rat Anti-Mouse CD273 antibody Cat. No. 565102; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD273 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of CD273 expression on mouse splenic leucocytes dendritic cells . Mouse splenic leucocytes were cultured overnight with recombinant mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Panel or BD Horizon BUV395 Rat Anti-Mouse CD273 antibody Cat. No. 565102; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD273 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of CD273 expression on mouse splenic leucocytes dendritic cells . Mouse splenic leucocytes were cultured overnight with recombinant mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Panel or BD Horizon BUV395 Rat Anti-Mouse CD273 antibody Cat. No. 565102; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD273 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of CD273 expression on mouse splenic leucocytes dendritic cells . Mouse splenic leucocytes were cultured overnight with recombinant mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Panel or BD Horizon BUV395 Rat Anti-Mouse CD273 antibody Cat. No. 565102; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD273 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of CD273 expression on mouse splenic leucocytes dendritic cells . Mouse splenic leucocytes were cultured overnight with recombinant mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c antibody Cat. No. 550261/561119 and either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Panel or BD Horizon BUV395 Rat Anti-Mouse CD273 antibody Cat. No. 565102; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD273 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.