
565974 · 25 ug
BDB Bioscience · Cat: 565974
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 and BD Horizon BUV395 Rat Anti-Mouse CD4 Cat. No. 563790/565794 antibodies. The two-color fluorescence dot plot shows the correlated expression patterns of CD4 versus CD3e for gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 and BD Horizon BUV395 Rat Anti-Mouse CD4 Cat. No. 563790/565794 antibodies. The two-color fluorescence dot plot shows the correlated expression patterns of CD4 versus CD3e for gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 and BD Horizon BUV395 Rat Anti-Mouse CD4 Cat. No. 563790/565794 antibodies. The two-color fluorescence dot plot shows the correlated expression patterns of CD4 versus CD3e for gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.