BD HorizonTM BUV395 Rat Anti-Mouse CD4

Description

Multiparameter flow cytometric analysis of CD4 expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific . Multiparameter flow cytometric analysis of CD4 expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific . Multiparameter flow cytometric analysis of CD4 expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific . Show More Multiparameter flow cytometric analysis of CD4 expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific . Show More Multiparameter flow cytometric analysis of CD4 expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific . Multiparameter flow cytometric analysis of CD4 expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific .