BD HorizonTM BUV395 Rat Anti-Mouse CD62L (L-Selectin)

Description

Multiparameter flow cytometric analysis of CD62L L-Selectin expression on viable Mouse splenic leucocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse CD62L L-Selectin antibody Cat. No. 569400; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD62L L-Selectin or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD62L L-Selectin expression on Unstimulated and Stimulated viable Mouse bone marrow cells. Mouse bone marrow cells were left untreated Unstimulated; Left Plot or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Stimulated Right Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; dashed line histogram or BD Horizon BUV395 Rat Anti-Mouse CD62L L-Selectin antibody Cat. No. 569400; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD62L L-Selectin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD62L L-Selectin expression on viable Mouse splenic leucocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse CD62L L-Selectin antibody Cat. No. 569400; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD62L L-Selectin or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD62L L-Selectin expression on Unstimulated and Stimulated viable Mouse bone marrow cells. Mouse bone marrow cells were left untreated Unstimulated; Left Plot or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Stimulated Right Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; dashed line histogram or BD Horizon BUV395 Rat Anti-Mouse CD62L L-Selectin antibody Cat. No. 569400; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD62L L-Selectin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD62L L-Selectin expression on viable Mouse splenic leucocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; Left Plot or BD Horizon BUV395 Rat Anti-Mouse CD62L L-Selectin antibody Cat. No. 569400; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD62L L-Selectin or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD62L L-Selectin expression on Unstimulated and Stimulated viable Mouse bone marrow cells. Mouse bone marrow cells were left untreated Unstimulated; Left Plot or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Stimulated Right Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either BD Horizon BUV395 Rat IgG2a, kappa Isotype Control Cat. No. 563556; dashed line histogram or BD Horizon BUV395 Rat Anti-Mouse CD62L L-Selectin antibody Cat. No. 569400; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD62L L-Selectin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.