BD HorizonTM BUV395 Rat Anti-Mouse IL-17A

Description

Flow cytometric analysis of IL-17A-producing mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Rat IgG1 kappa Isotype Control Cat. No. 564059; Left Panel or BD Horizon BUV395 Rat Anti-Mouse IL-17A antibody Cat. No. 565246; Right Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color contour plots showing the expression of IL-17A or Ig Isotype control staining versus Autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of IL-17A-producing mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Rat IgG1 kappa Isotype Control Cat. No. 564059; Left Panel or BD Horizon BUV395 Rat Anti-Mouse IL-17A antibody Cat. No. 565246; Right Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color contour plots showing the expression of IL-17A or Ig Isotype control staining versus Autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of IL-17A-producing mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Rat IgG1 kappa Isotype Control Cat. No. 564059; Left Panel or BD Horizon BUV395 Rat Anti-Mouse IL-17A antibody Cat. No. 565246; Right Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color contour plots showing the expression of IL-17A or Ig Isotype control staining versus Autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of IL-17A-producing mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Rat IgG1 kappa Isotype Control Cat. No. 564059; Left Panel or BD Horizon BUV395 Rat Anti-Mouse IL-17A antibody Cat. No. 565246; Right Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color contour plots showing the expression of IL-17A or Ig Isotype control staining versus Autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of IL-17A-producing mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Rat IgG1 kappa Isotype Control Cat. No. 564059; Left Panel or BD Horizon BUV395 Rat Anti-Mouse IL-17A antibody Cat. No. 565246; Right Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color contour plots showing the expression of IL-17A or Ig Isotype control staining versus Autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of IL-17A-producing mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Rat IgG1 kappa Isotype Control Cat. No. 564059; Left Panel or BD Horizon BUV395 Rat Anti-Mouse IL-17A antibody Cat. No. 565246; Right Panel using BD Biosciences Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color contour plots showing the expression of IL-17A or Ig Isotype control staining versus Autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.