BD HorizonTM BUV496 Mouse Anti-Mouse NK-1.1

Description

Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV711 Hamster Anti-Mouse CD3e antibody Cat. No. 563123 and with either BD Horizon BUV496 Mouse IgG2a, kappa Isotype Control Cat. No. 612957; Left Plot or BD Horizon BUV496 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569716; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV711 Hamster Anti-Mouse CD3e antibody Cat. No. 563123 and with either BD Horizon BUV496 Mouse IgG2a, kappa Isotype Control Cat. No. 612957; Left Plot or BD Horizon BUV496 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569716; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV711 Hamster Anti-Mouse CD3e antibody Cat. No. 563123 and with either BD Horizon BUV496 Mouse IgG2a, kappa Isotype Control Cat. No. 612957; Left Plot or BD Horizon BUV496 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569716; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV711 Hamster Anti-Mouse CD3e antibody Cat. No. 563123 and with either BD Horizon BUV496 Mouse IgG2a, kappa Isotype Control Cat. No. 612957; Left Plot or BD Horizon BUV496 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569716; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV711 Hamster Anti-Mouse CD3e antibody Cat. No. 563123 and with either BD Horizon BUV496 Mouse IgG2a, kappa Isotype Control Cat. No. 612957; Left Plot or BD Horizon BUV496 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569716; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV711 Hamster Anti-Mouse CD3e antibody Cat. No. 563123 and with either BD Horizon BUV496 Mouse IgG2a, kappa Isotype Control Cat. No. 612957; Left Plot or BD Horizon BUV496 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569716; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.