BD HorizonTM BUV496 Rat Anti-Mouse CD45

Description

Flow cytometric analysis of CD45 expression on viable Mouse splenic leukocytes. Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV496 Rat IgG2b, kappa Isotype Control Cat. No. 612954; dashed line histogram or BD Horizon BUV496 Rat Anti-Mouse CD45 antibody Cat. No. 569673; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD45 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45 expression on viable Mouse splenic leukocytes. Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV496 Rat IgG2b, kappa Isotype Control Cat. No. 612954; dashed line histogram or BD Horizon BUV496 Rat Anti-Mouse CD45 antibody Cat. No. 569673; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD45 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45 expression on viable Mouse splenic leukocytes. Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV496 Rat IgG2b, kappa Isotype Control Cat. No. 612954; dashed line histogram or BD Horizon BUV496 Rat Anti-Mouse CD45 antibody Cat. No. 569673; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD45 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.