
612935 ·
BDB Bioscience · Cat: 612935
Reach 3+ verified distributors
Manufacturer product page
bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow Cytometric Analysis of Mouse CD45R/B220 or Human CD3 expression using BD Horizon BUV563 Streptavidin Left Panel - Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin Cat. No. 565765 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Right Panel - Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow Cytometric Analysis of Mouse CD45R/B220 or Human CD3 expression using BD Horizon BUV563 Streptavidin Left Panel - Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin Cat. No. 565765 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Right Panel - Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow Cytometric Analysis of Mouse CD45R/B220 or Human CD3 expression using BD Horizon BUV563 Streptavidin Left Panel - Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin Cat. No. 565765 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Right Panel - Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow Cytometric Analysis of Mouse CD45R/B220 or Human CD3 expression using BD Horizon BUV563 Streptavidin Left Panel - Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin Cat. No. 565765 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Right Panel - Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow Cytometric Analysis of Mouse CD45R/B220 or Human CD3 expression using BD Horizon BUV563 Streptavidin Left Panel - Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin Cat. No. 565765 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Right Panel - Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow Cytometric Analysis of Mouse CD45R/B220 or Human CD3 expression using BD Horizon BUV563 Streptavidin Left Panel - Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin Cat. No. 565765 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Right Panel - Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon BUV563 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.