BD HorizonTM BUV615 Mouse Anti-Human CD25

Description

Two-color flow cytometric analysis of CD25 expression on unstimulated peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody 555349/61840/561841 and either BD Horizon BUV615 Mouse IgG1, kappa Isotype Control Cat. No. 612986; Left Plot or BD Horizon BUV615 Mouse Anti-Human CD25 antibody Cat. No. 612996/612997; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD25 expression on activated peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon BUV615 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BUV615 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of CD25 expression on unstimulated peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody 555349/61840/561841 and either BD Horizon BUV615 Mouse IgG1, kappa Isotype Control Cat. No. 612986; Left Plot or BD Horizon BUV615 Mouse Anti-Human CD25 antibody Cat. No. 612996/612997; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD25 expression on activated peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon BUV615 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BUV615 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of CD25 expression on unstimulated peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody 555349/61840/561841 and either BD Horizon BUV615 Mouse IgG1, kappa Isotype Control Cat. No. 612986; Left Plot or BD Horizon BUV615 Mouse Anti-Human CD25 antibody Cat. No. 612996/612997; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD25 expression on activated peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon BUV615 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BUV615 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.