BD HorizonTM BUV615 Mouse Anti-Human CD279 (PD-1)

Description

Multiparameter flow cytometric analysis of CD279 PD-1 expression on human peripheral blood leucocytes. Whole blood was stained with FITC Mouse Anti-Human CD3 antibody Cat. No. 561806/561807/555332 and either BD Horizon BUV615 Mouse IgG1, kappa Isotype Control Cat. No. 612986; Left Plots or BD Horizon BUV615 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 612991; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus side light-scatter SSC signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes Upper Plots . The bivariate pseudocolor density plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Lower Plots . Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD279 PD-1 expression on human peripheral blood leucocytes. Whole blood was stained with FITC Mouse Anti-Human CD3 antibody Cat. No. 561806/561807/555332 and either BD Horizon BUV615 Mouse IgG1, kappa Isotype Control Cat. No. 612986; Left Plots or BD Horizon BUV615 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 612991; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus side light-scatter SSC signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes Upper Plots . The bivariate pseudocolor density plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Lower Plots . Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD279 PD-1 expression on human peripheral blood leucocytes. Whole blood was stained with FITC Mouse Anti-Human CD3 antibody Cat. No. 561806/561807/555332 and either BD Horizon BUV615 Mouse IgG1, kappa Isotype Control Cat. No. 612986; Left Plots or BD Horizon BUV615 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 612991; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus side light-scatter SSC signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes Upper Plots . The bivariate pseudocolor density plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Lower Plots . Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software.