BD HorizonTM BUV615 Rat Anti-Mouse CD8a

Description

Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BUV615 Rat IgG2a, kappa Isotype Control Cat. No. 613005; Left Plot or BD Horizon BUV615 Rat Anti-Mouse CD8a antibody Cat. No. 613004; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable 7-AAD negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BUV615 Rat IgG2a, kappa Isotype Control Cat. No. 613005; Left Plot or BD Horizon BUV615 Rat Anti-Mouse CD8a antibody Cat. No. 613004; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable 7-AAD negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BUV615 Rat IgG2a, kappa Isotype Control Cat. No. 613005; Left Plot or BD Horizon BUV615 Rat Anti-Mouse CD8a antibody Cat. No. 613004; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable 7-AAD negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BUV615 Rat IgG2a, kappa Isotype Control Cat. No. 613005; Left Plot or BD Horizon BUV615 Rat Anti-Mouse CD8a antibody Cat. No. 613004; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable 7-AAD negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BUV615 Rat IgG2a, kappa Isotype Control Cat. No. 613005; Left Plot or BD Horizon BUV615 Rat Anti-Mouse CD8a antibody Cat. No. 613004; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable 7-AAD negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BUV615 Rat IgG2a, kappa Isotype Control Cat. No. 613005; Left Plot or BD Horizon BUV615 Rat Anti-Mouse CD8a antibody Cat. No. 613004; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable 7-AAD negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.