BD HorizonTM BUV661 Rat Anti-Mouse CD150 (SLAM)

Description

Multiparameter flow cytometric analysis of CD150 SLAM expression by adult Mouse spleen cells and bone marrow hematopoietic stem cells. BALB/c Mouse spleen and bone marrow hematopoietic stem cells were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon BUV661 Rat IgG2a, kappa Isotype Control Cat. No. 612973 or BD Horizon BUV661 Rat Anti-Mouse CD150 SLAM antibody Cat. No. 567543 at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Left and Middle Plots: The spleen cells were further stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 . Bivariate pseudocolor density plots showing the correlated expression of Ig Isotype control staining Left Plot or CD150 SLAM Middle Plot versus CD45R/B220 were derived from gated events with the forward and side-light scatter characteristics of 7-AAD-negative viable splenic lymphoid cells. Right Plot: The fluorescence histogram showing the expression of Ig Isotype control staining solid line histogram or CD150 SLAM dashed line histogram was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow lymphoid cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD150 SLAM expression by adult Mouse spleen cells and bone marrow hematopoietic stem cells. BALB/c Mouse spleen and bone marrow hematopoietic stem cells were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon BUV661 Rat IgG2a, kappa Isotype Control Cat. No. 612973 or BD Horizon BUV661 Rat Anti-Mouse CD150 SLAM antibody Cat. No. 567543 at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Left and Middle Plots: The spleen cells were further stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 . Bivariate pseudocolor density plots showing the correlated expression of Ig Isotype control staining Left Plot or CD150 SLAM Middle Plot versus CD45R/B220 were derived from gated events with the forward and side-light scatter characteristics of 7-AAD-negative viable splenic lymphoid cells. Right Plot: The fluorescence histogram showing the expression of Ig Isotype control staining solid line histogram or CD150 SLAM dashed line histogram was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow lymphoid cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD150 SLAM expression by adult Mouse spleen cells and bone marrow hematopoietic stem cells. BALB/c Mouse spleen and bone marrow hematopoietic stem cells were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon BUV661 Rat IgG2a, kappa Isotype Control Cat. No. 612973 or BD Horizon BUV661 Rat Anti-Mouse CD150 SLAM antibody Cat. No. 567543 at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Left and Middle Plots: The spleen cells were further stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 . Bivariate pseudocolor density plots showing the correlated expression of Ig Isotype control staining Left Plot or CD150 SLAM Middle Plot versus CD45R/B220 were derived from gated events with the forward and side-light scatter characteristics of 7-AAD-negative viable splenic lymphoid cells. Right Plot: The fluorescence histogram showing the expression of Ig Isotype control staining solid line histogram or CD150 SLAM dashed line histogram was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow lymphoid cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD150 SLAM expression by adult Mouse spleen cells and bone marrow hematopoietic stem cells. BALB/c Mouse spleen and bone marrow hematopoietic stem cells were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon BUV661 Rat IgG2a, kappa Isotype Control Cat. No. 612973 or BD Horizon BUV661 Rat Anti-Mouse CD150 SLAM antibody Cat. No. 567543 at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Left and Middle Plots: The spleen cells were further stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 . Bivariate pseudocolor density plots showing the correlated expression of Ig Isotype control staining Left Plot or CD150 SLAM Middle Plot versus CD45R/B220 were derived from gated events with the forward and side-light scatter characteristics of 7-AAD-negative viable splenic lymphoid cells. Right Plot: The fluorescence histogram showing the expression of Ig Isotype control staining solid line histogram or CD150 SLAM dashed line histogram was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow lymphoid cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD150 SLAM expression by adult Mouse spleen cells and bone marrow hematopoietic stem cells. BALB/c Mouse spleen and bone marrow hematopoietic stem cells were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon BUV661 Rat IgG2a, kappa Isotype Control Cat. No. 612973 or BD Horizon BUV661 Rat Anti-Mouse CD150 SLAM antibody Cat. No. 567543 at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Left and Middle Plots: The spleen cells were further stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 . Bivariate pseudocolor density plots showing the correlated expression of Ig Isotype control staining Left Plot or CD150 SLAM Middle Plot versus CD45R/B220 were derived from gated events with the forward and side-light scatter characteristics of 7-AAD-negative viable splenic lymphoid cells. Right Plot: The fluorescence histogram showing the expression of Ig Isotype control staining solid line histogram or CD150 SLAM dashed line histogram was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow lymphoid cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD150 SLAM expression by adult Mouse spleen cells and bone marrow hematopoietic stem cells. BALB/c Mouse spleen and bone marrow hematopoietic stem cells were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon BUV661 Rat IgG2a, kappa Isotype Control Cat. No. 612973 or BD Horizon BUV661 Rat Anti-Mouse CD150 SLAM antibody Cat. No. 567543 at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Left and Middle Plots: The spleen cells were further stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553088/553087/561877 . Bivariate pseudocolor density plots showing the correlated expression of Ig Isotype control staining Left Plot or CD150 SLAM Middle Plot versus CD45R/B220 were derived from gated events with the forward and side-light scatter characteristics of 7-AAD-negative viable splenic lymphoid cells. Right Plot: The fluorescence histogram showing the expression of Ig Isotype control staining solid line histogram or CD150 SLAM dashed line histogram was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow lymphoid cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.